Temperature-programmed high performance liquid chromatography separation of mono- and divinyl chlorophyll forms from marine phytoplankton
Chromatographia, ISSN: 0009-5893, Vol: 41, Issue: 5-6, Page: 539-543
1995
- 5Citations
- 16Captures
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Article Description
Divinyl chlorophyll a (a) and divinyl chlorophyll b (b) are chemotaxonomic marker pigments for the marine prochlorophytes, and can be used to study their distribution in marine samples. In this paper we report a baseline resolution of mono- and divinyl forms of chlorophylls a and b employing polymeric ODS stationary phases at sub-ambient temperatures. The simultaneous resolution of mono- and divinyl forms of chlorophylls a and b, chlorophylls c, c, c, Mg 3,8-divinylphaeoporphyrin a monomethyl ester (MgDVP), and phytol-substituted chlorophylls c was achieved when a temperature step gradient was employed during the analysis. An example is given of the utility of the protocol in oceanic field samples. © 1995 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=51649136183&origin=inward; http://dx.doi.org/10.1007/bf02688083; http://link.springer.com/10.1007/BF02688083; http://link.springer.com/content/pdf/10.1007/BF02688083; http://link.springer.com/content/pdf/10.1007/BF02688083.pdf; http://link.springer.com/article/10.1007/BF02688083/fulltext.html; https://dx.doi.org/10.1007/bf02688083; https://link.springer.com/article/10.1007/BF02688083
Springer Science and Business Media LLC
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