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Isolation of the genomic clone for pathogenesis-related protein 1a from Nicotiana tabacum cv. Xanthi-nc

Plant Molecular Biology, ISSN: 0167-4412, Vol: 11, Issue: 2, Page: 89-94
1988
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We describe the isolation of the chromosomal gene for pathogenesis-related protein 1a from Nicotiana tabacum. A 2 kb fragment containing the PR-1a gene as well as 5′ and 3′ flanking DNA has been sequenced and the transcriptional start site has been determined by primer extension and S1 nuclease mapping. 80% of the protein sequence from purified PR-1a and 20% of the sequence of purified PR-1b has also been determined and used to verify the nomenclature established for the PR-1 cDNAs. © 1988 Kluwer Academic Publishers.

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