PlumX Metrics
Embed PlumX Metrics

Shoot tips of wheat as an alternative source for regenerable embryogenic callus cultures

Plant Cell, Tissue and Organ Culture, ISSN: 0167-6857, Vol: 44, Issue: 3, Page: 183-188
1996
  • 33
    Citations
  • 0
    Usage
  • 11
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    33
    • Citation Indexes
      33
  • Captures
    11

Article Description

Shoot tips of Triticum aestivum L. cvs. Turbo and Nandu, both summer wheat varieties, were excised from 4 and 10 day-old seedlings, and used for induction of embryogenic callus. A modified L3 medium, supplemented with 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) for culture initiation, and 5 μM 2,4-D for subculturing, was optimal; 90% of 4 day-old 'Turbo' seedlings formed embryogenic callus. Optimal plant regeneration was achieved from callus incubated on a modified MS medium without 2,4-D, but supplemented with 2.22 μM 6-benzylaminopurine and 0.27 μM naphthaleneacetic acid. Plantlets formed via embryogenesis from all embryogenic 'Turbo' calli initiated from 4 day-old explants, with a mean number of 8 regenerants per explant. Regeneration occured via embryogenesis only. Results obtained using 'Nandu' were within the same range.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know