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Cloning of E. coli penicillin G acylase gene with mini-Mu containing a plasmid replicon

Biotechnology Letters, ISSN: 0141-5492, Vol: 17, Issue: 1, Page: 13-18
1995
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An in vivo cloning system based on mini-Mu derivatives was used for cloning of E. coli penicillin G acylase gene (pac). We have constructed several recombinant clones producing penicillin G acylase and some of them exhibit approximately two times higher activity than original strains. © 1995 Chapman & Hall.

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