Immunolocalization of ubiquitin in degenerating insect flight muscle
The Histochemical Journal, ISSN: 0018-2214, Vol: 26, Issue: 4, Page: 298-305
1994
- 15Citations
- 24Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations15
- Citation Indexes15
- 15
- CrossRef11
- Captures24
- Readers24
- 24
Article Description
Ubiquitin was localized by immunofluorescence microscopy during post-mating histolysis of fibrillar flight muscle in female fire ants, Solenopsis spp. Normal muscles, as well as histolysing muscles from artificially inseminated and haemolymph-injected females contained ubiquitin in association with nuclei, Z-lines, myofilaments and mitochondria. However, the density of the ubiquitin immunoreaction was markedly increased in the nuclei, Z-lines and mitochondria of degenerating tissues 6, 12 and 24 h post treatment. At these times the heaviest immunoreactivity for ubiquitin was seen in association with the nuclei, Z-lines and mitochondria. Immuno-controls, incubated in the absence of the primary antibody, showed no similar immunostaining. When insemination was preceded by the injection of actinomycin D, muscle degradation was significantly depressed after a 24-h period. Also, ubiquitin immunofluorescence was markedly reduced in tissues pre-treated with actinomycin D. These observations suggest that insemination increases the ubiquitination of specific myofibrillar proteins destined for degradation. © 1994 Chapman & Hall.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0028344824&origin=inward; http://dx.doi.org/10.1007/bf00157762; http://www.ncbi.nlm.nih.gov/pubmed/8040003; http://link.springer.com/10.1007/BF00157762; https://dx.doi.org/10.1007/bf00157762; https://link.springer.com/article/10.1007/BF00157762; http://www.springerlink.com/index/10.1007/BF00157762; http://www.springerlink.com/index/pdf/10.1007/BF00157762
Springer Nature
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