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Inheritance of mitochondrial DNA in lentil (Lens culinaris Medik.)

Theoretical and Applied Genetics, ISSN: 0040-5752, Vol: 89, Issue: 2-3, Page: 206-210
1994
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Restriction fragment analysis was used to examine the inheritance of lentil mitochondrial DNA (mtDNA) in F and F progeny from intrasubspecific (Lens culinaris ssp. culinaris) crosses and in F progeny from intersubspecific (Lens culinaris ssp. orientalis x L. culinaris ssp. culinaris) crosses. Southern blots of digested parental and progeny DNA were hybridized to heterologous maize mtDNA probes specific to coxI and atp6 genes. Two restriction fragment polymorphisms separated L.c. ssp. culinaris 'Laird' and 'Eston' from L.c. ssp. culinaris 'ILL5588', and one restriction fragment polymorphism distinguished L.c. ssp. culinaris 'Laird' and 'Eston' from L.c. ssp. orientalis 'LO4'. Twelve of 13 f progeny and all F progeny from the intrasubspecific crosses, and all F progeny from intersubspecific crosses had only maternal mtDNA restriction fragments. One f plant from an 'Eston' x 'ILL5588' cross inherited mtDNA fragments from both parents. Nuclear DNA inheritance was biparental in all F progeny. © 1994 Springer-Verlag.

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