Proline production via the arginine biosynthetic pathway: transfer of regulatory mutations of arginine biosynthesis into a proline-producing strain of Serratia marcescens

Proline production via a part of the arginine biosynthetic pathway was examined. About 20 mg/ml of l-proline was produced by using arginine biosynthetic enzymes. Accordingly, three mutations of arginine biosynthesis, namely, derepression of arginine biosynthetic enzymes (assigned by argR2), feedback inhibition-resistant N-acetylglutamate synthase (assigned by argA2) and defectiveness in N-acetylornithine aminotransferase (assigned by argD) were introduced by three transductional crosses into a proline-producing strain which produced about 55 mg/ml of l-proline. The constructed strain produced 62 mg/ml of l-proline, although about 10 mg/ml of l-arginine and 1 mg/ml of N-acetylglutamate-γ-semialdehyde were produced as by-products. © 1986 Springer-Verlag.