Novel plasmid marker rescue transformation system for molecular cloning in Bacillus subtilis enabling direct selection of recombinants
MGG Molecular & General Genetics, ISSN: 0026-8925, Vol: 223, Issue: 2, Page: 185-191
1990
- 15Citations
- 17Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations15
- Citation Indexes15
- 15
- CrossRef10
- Captures17
- Readers17
- 17
Article Description
A versatile plasmid marker rescue transformation system was developed for homology-facilitated cloning in Bacillus subtilis. It is based on the highly efficient host-vector system 6GM15-pHPS9, which allows the direct selection of recombinants by means of β-galactosidase α-complementation. The system offers several advantages over previously described cloning systems: (1) the convenient direct selection of recombinants; (2) the ability to effectively transform B. subtilis competent cells with plasmid monomers, which allows the forced cloning of DNA fragments with high efficiency; (3) the availability of 6 unique target sites, which can be used for direct clone selection, SphI, NdeI, NheI, BamHI, SmaI and EcoRI; and (4) the rapid segregational loss of the helper plasmid from the transformed cells. © 1990 Springer-Verlag.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025054676&origin=inward; http://dx.doi.org/10.1007/bf00265052; http://www.ncbi.nlm.nih.gov/pubmed/2123518; http://link.springer.com/10.1007/BF00265052; http://www.springerlink.com/index/10.1007/BF00265052; http://www.springerlink.com/index/pdf/10.1007/BF00265052; https://dx.doi.org/10.1007/bf00265052; https://link.springer.com/article/10.1007/BF00265052
Springer Science and Business Media LLC
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