Modulation of glutamine synthetase gene expression in tobacco by the introduction of an alfalfa glutamine synthetase gene in sense and antisense orientation: molecular and biochemical analysis
MGG Molecular & General Genetics, ISSN: 0026-8925, Vol: 236, Issue: 2-3, Page: 315-325
1993
- 62Citations
- 14Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations62
- Citation Indexes62
- 62
- CrossRef43
- Captures14
- Readers14
- 14
Article Description
A glutamine synthetase (GS) cDNA isolated from an alfalfa cell culture cDNA library was found to represent a cytoplasmic GS. The full-length alfalfa GS coding sequence, in both sense and antisense orientation and under the transcriptional control of the cauliflower mosaic virus 35S promoter, was introduced into tobacco. Leaves of tobacco plants transformed with the sense construct contained greatly elevated levels of GS transcript and GS polypeptide which assembled into active enzyme. Leaves of the plants transformed with the antisense GS, construct showed a significant decrease in the level of both GS, and GS, polypeptides and GS activity, but did not show any significant decrease in the level of endogenous GS mRNA. We have proposed that antisense inhibition using a heterologous antisense GS RNA occurs at the level of translation. Our results also suggest that the post-translational assembly of GS subunits into a holoenzyme requires an additional factor(s) and is under regulatory control. © 1993 Springer-Verlag.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0027402664&origin=inward; http://dx.doi.org/10.1007/bf00277128; http://www.ncbi.nlm.nih.gov/pubmed/8094885; http://link.springer.com/10.1007/BF00277128; https://dx.doi.org/10.1007/bf00277128; https://link.springer.com/article/10.1007/BF00277128; http://www.springerlink.com/index/10.1007/BF00277128; http://www.springerlink.com/index/pdf/10.1007/BF00277128
Springer Science and Business Media LLC
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