Protein A radio-assay of H-Y antigen on human leukocytes using mouse and rat antisera and monoclonal antibodies
Human Genetics, ISSN: 0340-6717, Vol: 65, Issue: 2, Page: 190-194
1983
- 8Citations
- 1Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations8
- Citation Indexes8
- CrossRef8
- Captures1
- Readers1
Article Description
The presence of H-Y antigen on human leukocytes was investigated using a protein A radio-assay. H-Y antigen could be demonstrated on male cells using either conventional H-Y antisera produced in mice and rats, or monoclonal H-Y antibodies. With mouse antiserum and IgG-type monoclonal antibody the reaction was male-specific using a single antibody. The reaction obtained with rat antiserum was enhanced by the application of a second antibody (rabbit anti-mouse IgG). This technique provides a rapid, simple, objective, and semiquantitative method for the determination of cellular H-Y antigen, the results being expressed as radioactivity bound to the test cells and thus being independent of human observation. It requires only 10-20 ml of blood and small quantities of antiserum or antibody. © 1983 Springer-Verlag.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0021073359&origin=inward; http://dx.doi.org/10.1007/bf00286662; http://www.ncbi.nlm.nih.gov/pubmed/6654333; http://link.springer.com/10.1007/BF00286662; http://link.springer.com/content/pdf/10.1007/BF00286662; https://dx.doi.org/10.1007/bf00286662; https://link.springer.com/article/10.1007/BF00286662
Springer Nature
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