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Light- and fluorescence microscopic investigations on specific cells and structures in semithin sections of the connective- and nervous tissues of gastropods

Histochemie, ISSN: 0948-6143, Vol: 34, Issue: 2, Page: 177-190
1973
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In the present study specific cells and structures in the connective-and nervous tissues of some gastropods were demonstrated by light- and fluorescence techniques, performed on semithin sections of plastic embedded material. After dissolving the embedding medium (Epon or Styrene-methacrylate), the semithin sections are stainable by Gomori's Paraldehyde-Fuchsin, Aldehyde-Thionin, Alcian Blue and the fluorochromes Pseudoisocyanin and RF 500. Best results are obtained optically by staining with Pseudoisocyanin, because all fluorescing substances (i. a. the neurosecretory cells and fibres in the ganglia) appear on a practically dark background. For the first time, with Aldehyde-Thionin and Paraldehyde Fuchsin-but not with Pseudoisocyanin-neurosecretory fibres has been demonstrated in the cerebral commissure of Planorbarius corneus. The histochemical identifying of specific cells and structures in semithin sections by light- and especially fluorescence microscopic highly selective reactions gives the possibilities to analyse these target cells by electron microscopic investigations. © 1973 Springer-Verlag.

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