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The sensitivity of various dyes for detecting pancreatic beta granules in white mice during ontogenesis

Histochemie, ISSN: 0948-6143, Vol: 29, Issue: 1, Page: 54-64
1972
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Article Description

A comparative study of various methods for detecting secretory granules of pancreatic beta cells in fetal, young, and adult white mice was carried out in order to find out the most sensitive reaction. The influence of prior oxidation with performic acid or permanganate was tested. The following results were achieved: 1) With prior oxidation (permanganate) the pseudoisocyanins, without oxidation aldehyde fuchsin "II" and crotonaldehyde fuchsin exhibited the highest sensitivity for beta granules. The thiazine dyes and alcian blue showed a lower sensitivity, whereas the method for demonstration of SH- and SS-groups according to Barnett and Seligman (1954) was proved to be less sensitive. 2) Beta granules stained metachromatic with pseudoisocyanins were found to be anisotropic. © 1972 Springer-Verlag.

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