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Effect of formalin fixation on the dry mass of isolated rat liver nuclei

Histochemie, ISSN: 0948-6143, Vol: 29, Issue: 3, Page: 207-212
1972
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Effect of formalin fixation on the dry mass of isolated rat liver nuclei was studied by interference microscopy. It was found that a fixation time of 2-6 hrs prevents completely the elution of water-extractable material from nuclei. Nuclei unfixed, water-extracted for 4 hrs lose about 20% of their initial dry mass. Fixation time longer than 6 hrs resulted also in a decrease of dry mass of the nuclei. Liquid scintillation counting of C-formaldehyde served for finding the influence of bound formaldehyde on dry mass values. As calculated, after 2 hrs fixation with formalin the bound formaldehyde constitutes 3.6% of the dry mass of isolated untreated nuclei. © 1972 Springer-Verlag.

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