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Enhanced recombination between λp lac5 and mini-F-lac: The tra regulon is required for recombination enhancement

MGG Molecular & General Genetics, ISSN: 0026-8925, Vol: 193, Issue: 2, Page: 269-274
1984
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Article Description

F42 lac recombination with λp lac is normally 20-fold to 50-fold higher than recombination between λp lac and a chromosomal lac gene. Transductional crosses with λp lac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42 lac. © 1984 Springer-Verlag.

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