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Nonsense suppression context effects in Escherichia coli bacteriophage T4

MGG Molecular & General Genetics, ISSN: 0026-3925, Vol: 207, Issue: 2-3, Page: 517-518
1987
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Nonsense suppression by supE44 has been examined in a collection of 14 T4 gene 22 and gene 23 UAG mutants, for which the precise gene location is known. In concordance with previous studies, UAG followed by a pyrimidine was inefficiently suppressed. However, among positions with similar 3′ nucleotides, there was considerable variation in suppression efficiency. The competition between supE44 and Release Factor 1 (RF1) was also investigated following the introduction of a multicopy RF1 plasmid. An inverse relationship between the efficiency of suppression and RF1 competition was observed. © 1987 Springer-Verlag.

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