Homology-facilitated plasmid transfer in Haemophilus influenzae
MGG Molecular & General Genetics, ISSN: 0026-3925, Vol: 203, Issue: 2, Page: 288-295
1986
- 17Citations
- 3Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations17
- Citation Indexes17
- CrossRef17
- 12
- Captures3
- Readers3
Article Description
The 8 kbp plasmid pAT4 transformed Haemophilus influenzae Rd cells at low frequencies. Transformation was increased up to 100 times, however, when the recipient cells carried a DNA segment in either their chromosome or in a resident plasmid that was homologous to at least part of plasmid pAT4. Linearized plasmid DNA molecules did not transform cells without DNA homology; they efficiently transformed homology recipients, but only when the cuts had been made in the region of shared homology. In most cases examined the circular donor plasmid had been reconstituted from the transforming DNA; in some cases the reconstituted plasmid carried a mutation initially present in the recipient chromosome, provided the transforming plasmid had been linearized in the region of shared homology. Plasmid reconstitution was not observed in recA1 cells. We conclude that homology-facilitated plasmid transformation (transfer) is similar to that reported for Bacillus subtilis and Streptococcus pneumoniae. © 1986 Springer-Verlag.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0022711021&origin=inward; http://dx.doi.org/10.1007/bf00333968; http://www.ncbi.nlm.nih.gov/pubmed/3016481; http://link.springer.com/10.1007/BF00333968; https://dx.doi.org/10.1007/bf00333968; https://link.springer.com/article/10.1007/BF00333968; http://www.springerlink.com/index/10.1007/BF00333968; http://www.springerlink.com/index/pdf/10.1007/BF00333968
Springer Science and Business Media LLC
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