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An essential gene for replication of the mini-F plasmid from origin I

MGG Molecular & General Genetics, ISSN: 0026-8925, Vol: 196, Issue: 1, Page: 59-63
1984
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We constructed a series of defective mini-F plasmids, which have deletion(s) in the replication origin I and/or origin II, and their derivatives, which do not produce F3 protein, by insertion of the XhoI fragment of Tn5 into the XhoI site at 41.0 F (kilobases on the coordinate map of F-plasmid). Using these mutant mini-F plasmids, we found that F3 protein is essential for the replication of mini-F from origin I, but not from origin II. © 1984 Springer-Verlag.

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