Amplification of plasmid copy number by thymidine kinase expression in Saccharomyces cerevisiae
MGG Molecular & General Genetics, ISSN: 0026-8925, Vol: 211, Issue: 1, Page: 155-159
1988
- 10Citations
- 9Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations10
- Citation Indexes10
- CrossRef10
- 10
- Captures9
- Readers9
Article Description
A 2 μm circle-based chimaeric plasmid containing the yeast LEU2 and the Herpes Simplex Virus type 1 thymidine kinase (HSV-1 TK) genes was constructed. Transformants grown under selective conditions for the LEU2 gene harboured the plasmid at about 15 copies per cell whilst selection for the HSV-1 TK gene led to an increase to about 100 copies per cell. Furthermore, the plasmid copy number could be controlled by the stringency of selection for the TK gene, and the increase in TK gene dosage was reflected in an increase in intracellular thymidine kinase activity. The mitotic stability of the plasmid in "high-copy" and "low-copy" number cells was determined. "High-copy" number cells showed a greater mitotic stability. The relationship of TK expression to plasmid copy number may be useful for the isolation of plasmid copy number mutants in yeast and the control of heterologous gene expression. © 1988 Springer-Verlag.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0023878344&origin=inward; http://dx.doi.org/10.1007/bf00338407; http://www.ncbi.nlm.nih.gov/pubmed/2830462; http://link.springer.com/10.1007/BF00338407; https://dx.doi.org/10.1007/bf00338407; https://link.springer.com/article/10.1007/BF00338407; http://www.springerlink.com/index/10.1007/BF00338407; http://www.springerlink.com/index/pdf/10.1007/BF00338407
Springer Science and Business Media LLC
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