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The cell biology of Drosophila wing metamorphosis in vitro

Roux's Archives of Developmental Biology, ISSN: 0930-035X, Vol: 196, Issue: 3, Page: 191-201
1987
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We have examined the metamorphosis of the wing imaginal disc of Drosophila during culture in vitro in the continuous presence of 20-hydroxy ecdysone (0.1 μg/ ml). We find that the sequence of cellular changes in the wing blade during culture closely match those occurring in situ, involving two periods at which the dorsal and ventral surfaces are joined only by cell processes containing trans-alar microtubule arrays. Good pupal and imaginal cuticle secretion is found in this system. © 1987 Springer-Verlag.

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