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Partial purification and some properties of an alkaline cellulase from an alkalophilic Bacillus sp.

World Journal of Microbiology and Biotechnology, ISSN: 0959-3993, Vol: 12, Issue: 5, Page: 525-529
1996
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Article Description

A newly isolated Bacillus species, which grew optimally at 30°6 and pH 10, produced a carboxymethylcellulase in a medium containing 10 g CM- cellulose/I. The enzyme, when partially purified by gel filtration, had a mass of about 29 kDa as determined by both SDS-PAGE and gel filtration chromatography. It was optimally active at pH 9.5 and 40°C, and was stable from pH 7 to 11 at 4°C for 24 h. The enzyme was stimulated by Ca (1 mM) but was completely inhibited by Hg (1 mM). Neither EDTA nor EGTA (10 mM) affected the activity.

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