Quantitative I-autoradiography of individual cells
Histochemistry, ISSN: 0301-5564, Vol: 43, Issue: 1, Page: 33-49
1975
- 11Citations
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations11
- Citation Indexes11
- CrossRef11
Article Description
Iodine 125, an emitter of β-radiation with an energy lying between that of tritium and carbon-14, is investigated for its applicability in quantitative autoradiography. Absorption and geometric factors of radiation are elucidated. From this, appropriate measuring conditions are derived. The simultaneous exposure of radioactive standard sources permits the evaluation of absolute amounts of radioactivity. Standard cells with labelled membranes are a suitable source of reference taking into account the physical properties of the isotope. Sheep red blood cells are examined for their suitability as standard cells after enzymatic radioiodination. The absolute number of antigenic substances on the surface of single cells is obtained by determining the specific activity of the labelled antibody molecules, and by measuring the silver grain densities of the cells under investigation and of the standard cells. The radioactivity per standard cell can be assessed by conventional procedures. The new method is applied to the quantification of membrane-bound immunoglobulin molecules of the IgG-type on single human lymphocytes. The determination of an immunologic saturation of the labelled antibody is essential for this purpose. On the lymphocytes of a normal person and of a patient with chronic lymphatic leukaemia quite different amounts of immunoglobulins have been evaluated. © 1975 Springer-Verlag.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0016590852&origin=inward; http://dx.doi.org/10.1007/bf00490152; http://www.ncbi.nlm.nih.gov/pubmed/1141024; http://link.springer.com/10.1007/BF00490152; http://www.springerlink.com/index/pdf/10.1007/BF00490152; https://dx.doi.org/10.1007/bf00490152; https://link.springer.com/article/10.1007/BF00490152; http://www.springerlink.com/index/10.1007/BF00490152
Springer Nature
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