Detection of elastin by immunoelectronmicroscopy - A comparison of different procedures
Histochemistry, ISSN: 0301-5564, Vol: 87, Issue: 6, Page: 573-578
1987
- 10Citations
- 4Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations10
- Citation Indexes10
- CrossRef10
- 10
- Captures4
- Readers4
Article Description
Elastin components have been identified in chick aorta by different immunoelectronmicroscopic procedures (peroxidase-antiperoxidase, immunoferritin and immunogold) using affinity purified antibodies to chick tropoelastin. The PAP method used in a preembedding procedure stained the outer portion of amorphous elastin and the microfibrils very intensively. The surface of the cells was also slightly stained. On the contrary immunogold labelling on Epon or Lowicryl embedded sections produced a strong decoration only of amorphous elastin, while microfibrils remained almost completely unlabelled. The result is not due to loss of antigenicity of microfibrils during embedding, since similar data were obtained with immunoferritin in a preembedding procedure. Experiments performed under different stringency conditions showed that the products of the peroxidase reaction diffuse and redistribute in the tissue, indicating that the positive staining of microfibrils and cell surface is an artifact. The value of different immunological reagents and procedures in studying the fine mapping of clastin components is discussed. © 1987 Springer-Verlag.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0023493967&origin=inward; http://dx.doi.org/10.1007/bf00492473; http://www.ncbi.nlm.nih.gov/pubmed/3692923; http://link.springer.com/10.1007/BF00492473; http://www.springerlink.com/index/pdf/10.1007/BF00492473; http://www.springerlink.com/index/10.1007/BF00492473; https://dx.doi.org/10.1007/bf00492473; https://link.springer.com/article/10.1007/BF00492473
Springer Nature
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