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Studies on dentinogenesis in the rat - Ethane-1-hydroxy-1,1-diphosphonate (EHDP) inhibits crystal growth in predentin calcification

Virchows Archiv Abteilung A Pathologische Anatomie, ISSN: 0945-6317, Vol: 360, Issue: 4, Page: 315-325
1973
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The effects of ethane-1-hydroxy-1,1-diphosphonate (EHDP) in vivo on crystal growth in the early stages of predentin calcification were studied in newborn Sprague-Dawley rats after intraperitoneal injection of 0.1 ml physiological saline solution containing EHDP at a concentration of 100 or 500 μg/ml. The animals were given one injection per day for four consecutive days, thus receiving a total amount of 8 or 40 mg EHDP respectively. Control rats were injected with the same volume of physiologic saline alone. The following electron microscope observations were made: In dentin, the EHDP-treated rats were found to exhibit crystallites of smaller size than the normal controls. In the mineralization zone, most of the needle-like crystallites did not reach 20 Å in width. Their further growth was inhibited, only a few reaching 25 Å in width in the intertubular dentin. Within extracellular membrane-bound bodies or "dentinal globules" normally found in early formed predentin matrix, needle-like crystallites appeared before mineral deposits were visible in the predentin matrix. The crystallites had a paired appearance with a central electron-lucid core. The crystallite pattern was disturbed in EHDP-treated rats. Although less pronounced, similar changes were found also in rats given the lower dose of EHDP. © 1973 Springer-Verlag.

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