Electron-cytochemical localization of phospho(enol)pyruvate carboxylase (EC 4.1.1.31) in fungal cells
Histochemistry, ISSN: 1432-119X, Vol: 67, Issue: 3, Page: 257-265
1980
- 9Citations
- 1Captures
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Metrics Details
- Citations9
- Citation Indexes9
- CrossRef7
- Captures1
- Readers1
Article Description
New cytochemical method, based on biochemical experiments, was elaborated for the ultrastructural localization of phospho(enol)pyruvate carboxylase (EC 4.1.1.31). The procedure was used to study the saprophytic submerged mycelium of the ascomycetous fungus Claviceps purpurea Tul. producing clavine alkaloids. The pelleted mycelium was fixed in ice cold 3% glutaraldehyde in 50 mM cacodylate buffer pH 7.2 and washed repeatedly in the same cold buffer. The reaction mixture contained 100 mM Tris-HCl buffer pH 9.0, 10 mM phospho(enol)pyruvate, 30 mM sodium potassium tartrate, 3 mM Pb(NO), 60 mM MgCl and 30 mM NaHCO. Enzyme activity was localized in vacuoles, particularly inside lipid globules (spherosomes) and less frequently in membranous vesicles. Acetyl-CoA activated PEP-carboxylase both in cell free extracts and in the cytochemical staining. Aspartate inhibited the enzyme in the biochemical assay with coupled malate dehydrogenase system; the cytochemical reaction was not influenced, probably due to the interference of asparagine synthase (EC 6.3.1.1). © 1980 Springer-Verlag.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0019305346&origin=inward; http://dx.doi.org/10.1007/bf00692760; http://www.ncbi.nlm.nih.gov/pubmed/7399980; http://link.springer.com/10.1007/BF00692760; http://www.springerlink.com/index/pdf/10.1007/BF00692760; https://dx.doi.org/10.1007/bf00692760; https://link.springer.com/article/10.1007/BF00692760; http://www.springerlink.com/index/10.1007/BF00692760
Springer Science and Business Media LLC
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