Cloning and expression of a haloacid dehalogenase from Pseudomonas sp. strain 19 S
Biodegradation, ISSN: 0923-9820, Vol: 6, Issue: 3, Page: 217-222
1995
- 2Citations
- 4Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Article Description
A dehalogenase gene specifying the utilization of a variety of haloacids by Pseudomonas sp. Strain 19S has been cloned and expressed in E. coli. Our cloning strategy employed specific amplification of a fragment homologous to Pseudomonas dehalogenase gene by Polymerase Chain Reaction (PCR). The PCR amplicon successfully acted as a probe to detect the dehalogenase gene in the Southern Blot of the digested Pseudomonas total DNA. Corresponding fragments were cloned into pUC 18 vector and amplified in E. coli MV 1190. One clone with a substantial dehalogenation activity carried a recombinant plasmid containing a 5.5 kb insert. © 1995 Kluwer Academic Publishers.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029377138&origin=inward; http://dx.doi.org/10.1007/bf00700460; http://www.ncbi.nlm.nih.gov/pubmed/7579996; http://link.springer.com/10.1007/BF00700460; https://dx.doi.org/10.1007/bf00700460; https://link.springer.com/article/10.1007/BF00700460; http://www.springerlink.com/index/10.1007/BF00700460; http://www.springerlink.com/index/pdf/10.1007/BF00700460
Springer Science and Business Media LLC
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