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Purification and cellulosomal localization of Clostridium thermocellum mixed linkage β-glucanase LicB (1,3-1,4-β-D-glucanase)

Biotechnology Letters, ISSN: 0141-5492, Vol: 16, Issue: 1, Page: 29-34
1994
  • 27
    Citations
  • 0
    Usage
  • 7
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    27
    • Citation Indexes
      27
  • Captures
    7

Article Description

Clostridium thermocellum recombinant β-(1,3-1,4)-glucanase LicB was expressed in Escherichia coli K-12 and purified to electrophoretic homogeneity. Specific activity of the final preparation was 12,000 U/mg. Antibodies against recombinant LicB protein reacted with a specific band of purified C. thermocellum F7 cellulosomes, suggesting localization in the extracellular multienzyme complex. This indicates that the cellulosome takes part in the hydrolysis of other polysaccharides besides cellulose. © 1994 Kluwer Academic Publishers.

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