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Protein kinase C inhibition by calmodulin and its fragments

Journal of Protein Chemistry, ISSN: 0277-8033, Vol: 9, Issue: 4, Page: 467-473
1990
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Article Description

Inhibition of protein kinase C (PKC) by calmodulin is investigated and we describe the localization of inhibitory sequences within the calmodulin molecule. We present evidence that calmodulin inhibits PKC through an inhibition of the activation of PKC associated with lipid membranes: Binding of PKC to lipid vesicles is not affected, but activation is abolished. The potent calmodulin antagonist R24571 (calmidazol) did not affect the inhibition of PKC by calmodulin at concentrations up to 10 M. Two tryptic fragments of calmodulin were isolated which inhibited PKC. They were only slightly less potent than intact calmodulin with an IC of 6 μ M compared to 1 μ M of intact calmodulin. They were identified as Ser-Arg and His-Lys. Each of the inhibiting fragments contains an intact Ca-binding domain with complete helix-loop-helix structure ("EF hand"). Other calmodulin peptides showed only weak inhibitory activity. Both fragments did not stimulate cAMP phosphodiesterase even at concentrations 100-fold higher than the calmodulin concentration needed for maximal stimulation. None of the fragments acted as a calmodulin antagonist. © 1990 Plenum Publishing Corporation.

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