PlumX Metrics
Embed PlumX Metrics

A fibroblast heparan sulphate proteoglycan with a 70 kDa core protein is linked to membrane phosphatidylinositol

Glycoconjugate Journal, ISSN: 0282-0080, Vol: 7, Issue: 6, Page: 563-572
1990
  • 17
    Citations
  • 0
    Usage
  • 2
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

Here we present evidence that a fibroblast heparan sulphate proteoglycan of approx. 300 kDa and with a core protein of apparent molecular mass 70 kDa is covalently linked to the plasma membrane via a linkage structure involving phosphatidylinositol. Phosphatidylinositol-specific phospholipase C releases such a heparan sulphate proteoglycan only from cells labelled with [S]sulphate in the absence of serum. Cell cultures labelled with [H]myo-inositol in the absence or presence of serum produce a radiolabelled heparan sulphate proteoglycan which was purified by gel-permeation chromatography and ion-exchange chromatography on MonoQ. Digestion with heparan sulphate lyase and analysis by gel-permeation chromatography and sodium dodecylsulphate-polyacrylamide gel-electrophoresis revealed that theH-label is associated with a core protein of apparent mass 70 kDa. © 1990 Glycoconjugate Journal.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know