Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A/A difference
Glycoconjugate Journal, ISSN: 0282-0080, Vol: 7, Issue: 6, Page: 601-608
1990
- 13Citations
- 3Captures
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Metrics Details
- Citations13
- Citation Indexes13
- CrossRef13
- 12
- Captures3
- Readers3
Article Description
Total non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A/A phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A and A thrombocytes but the A individuals expressed at least ten times less A glycolipids compared to the A individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A and A individuals, while the type 2 chain A glycolipids appeared to be missing from the A thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A individuals and could not be detected in A individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region. © 1990 Glycoconjugate Journal.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025687409&origin=inward; http://dx.doi.org/10.1007/bf01189080; http://www.ncbi.nlm.nih.gov/pubmed/2136356; http://link.springer.com/10.1007/BF01189080; https://dx.doi.org/10.1007/bf01189080; https://link.springer.com/article/10.1007/BF01189080; http://www.springerlink.com/index/10.1007/BF01189080; http://www.springerlink.com/index/pdf/10.1007/BF01189080
Springer Science and Business Media LLC
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