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Synthetic oligonucleotide probes for detection of mercury-resistance genes in environmental freshwater microbial communities in response to pollutants

World Journal of Microbiology & Biotechnology, ISSN: 0959-3993, Vol: 8, Issue: 1, Page: 30-38
1992
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Mercury-resistance genes were detected by in situ hybridization using new synthetic oligonucleotide probes specific for merA and merB genes according to the published sequences of the corresponding enzymes. These DNA probes were used for the detection of specific mercury-resistant microorganisms isolated from the Rhine River which had been polluted 3 years previously in 1986. Mercuric reductase and organomercurial lyase genes persist in the bacterial genome even after the disappearance of the pollutant but are absent in axenic amoebae. A total of 49 bacterial isolates showed DNA homologies with theP-labelled DNA probes and 15 free-living amoebae were selected due to their harboured symbiotic mercury-resistant bacteria. © 1992 Rapid Communications of Oxford Ltd.

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