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H-UTP incorporation in polytene chromosomes of Chironomus permeabilized cells

Protoplasma, ISSN: 0033-183X, Vol: 101, Issue: 1-2, Page: 81-87
1979
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Article Description

A method is described that allows the cytological detection of the transcriptional activity of endogenous RNA polymerase in fixed Chironomus polytene chromosomes. H-UTP is preferentially incorporated onto nucleoli and Balbiani rings where transcription is specially high. The former incorporation was sensitive to actinomycin D while incorporation on Balbiani rings was sensitive to α-amanitin. The label pattern and frequency resemble those detected byH-uridine incorporation in the living state. © 1979 Springer-Verlag.

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