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Colocalization of luciferin binding protein and luciferase to the scintillons of Gonyaulax polyedra revealed by double immunolabeling after fast-freeze fixation

Protoplasma, ISSN: 0033-183X, Vol: 160, Issue: 2-3, Page: 159-166
1991
  • 41
    Citations
  • 0
    Usage
  • 12
    Captures
  • 3
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    41
    • Citation Indexes
      41
  • Captures
    12
  • Mentions
    3
    • References
      2
      • Wikipedia
        2
    • Blog Mentions
      1
      • Blog
        1

Article Description

Two proteins, Gonyaulax luciferase and the luciferin binding protein, are involved in the bioluminescent reaction of the unicellular marine alga Gonyaulax polyedra. Using antibodies raised separately against the purified proteins, their ultrastructural localizations were visualized by double immunogold labeling on sections after fast-freeze fixation, freeze-substitution and embedding in Epon or in LR White. Gold particles of two sizes attached to the secondary antibodies allowed the two primary antibodies to be distinguished. The two colocalized to cytoplasmic densifications (scintillons), which occurred in close association with the vacuolar membrane near the periphery of the cell. They also occurred in the cytoplasm of the Golgi area, either over densifications without associated membranes (prescintillons), or as very small colocalizations not associated with any evident cytoplasmic differentiation. No other site of colocalization was observed, thus unambiguously establishing the ultrastructural identity of the bioluminescent organelles. © 1991 Springer-Verlag.

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