Novel narrow-host-range vectors for direct cloning of foreign DNA in Pseudomonas
Current Microbiology, ISSN: 0343-8651, Vol: 28, Issue: 1, Page: 41-47
1994
- 4Citations
- 3Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations4
- Citation Indexes4
- CrossRef2
- Captures3
- Readers3
Article Description
Narrow-host-range vectors, based on an indigenous replicon and containing a multiple cloning site, have been constructed in a Pseudomonas host capable of growth on unusual substrates. The new cloning vectors yield sufficient amounts of DNA for preparative purposes and belong to an incompatibility group different from that of the incP and incQ broad-host-range vectors. One of these vectors, named pDB47F, was used to clone, directly in Pseudomonas, DNA fragments from Agrobacterium, Pseudomonas, and Rhizobium. A clone containing Agrobacterium and Km gene sequences was transformed with a higher efficiency than an RSF1010-derived vector (by as much as 1250-fold) in four out of five Pseudomonas strains tested. The considerable efficiency obtained with this system makes possible the direct cloning and phenotypic selection of foreign DNA in Pseudomonas. © 1994 Springer-Verlag New York Inc.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0028127868&origin=inward; http://dx.doi.org/10.1007/bf01575984; http://www.ncbi.nlm.nih.gov/pubmed/7764307; http://link.springer.com/10.1007/BF01575984; https://dx.doi.org/10.1007/bf01575984; https://link.springer.com/article/10.1007/BF01575984; http://www.springerlink.com/index/10.1007/BF01575984; http://www.springerlink.com/index/pdf/10.1007/BF01575984
Springer Science and Business Media LLC
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