The essentiality of His-47 and the N-terminal region for the binding of 8-anilinonaphthalene-1-sulfonate with Taiwan cobra phospholipase A
Journal of Protein Chemistry, ISSN: 0277-8033, Vol: 15, Issue: 3, Page: 255-260
1996
- 7Citations
- 1Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations7
- Citation Indexes7
- CrossRef4
- Captures1
- Readers1
Article Description
The binding of the apolar fluorescent dye 8-anilinonaphthalene-1- sulfonate (ANS) to Naja naja atra phospholipase A (PLA) as well as the enhancement of ANS fluorescence of the PLA-ANS complex decreased with increasing pH in a pH range from 3 to 9. These pH-dependent curves can be well interpreted as the perturbation of an ionizable group with pK value of 5.8, which was assigned as His-47 in the active site of PLA. The ionizable group with pK 5.8 was no longer observed after methylation of His-47, supporting the idea that the pH dependence of ANS binding arose from an electrostatic interaction between His-47 and the bound ANS. Removal of the N-terminal octapeptide of PLA caused a precipitous drop in the capability of PLA for binding with ANS and enhancing ANS fluorescence, reflecting that the integrity of the N terminal region was essential for maintaining the hydrophobic character of the ANS-binding site. However, the nonpolarity of the ANS-binding site in the N-terminus-removed derivative was still partially retained at low pH, but was completely lost at high pH. Evidently, the N-terminal region plays a more crucial role in ANS binding at high pH than at low pH. These results indicate thai hydrophobic interaction as well as electrostatic interaction are involved in the binding of ANS to PLA, and that the relative contributions of both interactions in ANS fluorescence enhancement may be different under different pH.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029949973&origin=inward; http://dx.doi.org/10.1007/bf01887113; http://www.ncbi.nlm.nih.gov/pubmed/8804572; http://link.springer.com/10.1007/BF01887113; http://www.springerlink.com/index/pdf/10.1007/BF01887113; https://link.springer.com/10.1007/BF01887113; http://www.springerlink.com/index/10.1007/BF01887113; https://dx.doi.org/10.1007/bf01887113; https://link.springer.com/article/10.1007/BF01887113
Springer Nature
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know