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A simple medium for the study of hepatocyte growth in culture under defined conditions

In Vitro Cellular & Developmental Biology, ISSN: 0883-8364, Vol: 24, Issue: 10, Page: 981-984
1988
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The combination (1:1) of Dulbecco's modified Eagle's medium and Waymouth's medium MAB 87/3 was found to provide favorable conditions for serum-free culture and growth of adult rat hepatocytes. In this simple medium, a majority of hepatocytes stimulated by epidermal growth factor plus insulin entered S phase and divided, with a normal (13 h) interval between DNA synthesis and cell division. The proliferative response did not require extra substratum or the presence of serum, even during cell isolation and plating. © 1988 Tissue Culture Association, Inc.

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