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Phage-Display Libraries of Murine and Human Antibody Fab Fragments

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology, ISSN: 1073-6085, Vol: 6, Issue: 3, Page: 287-310
1996
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Article Description

We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

Bibliographic Details

Jan Engberg; Peter Sejer Andersen; Lene K. Johansen; Bjarne Albrechtsen; Leif Kofoed Nielsen; Morten Dziegiel

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Immunology and Microbiology

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