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Quantification of high molecular weight (1→3)(1→4)-β-d-glucan using Calcofluor complex formation and flow injection analysis. II. determination of total β-glucan content of barley and malt

Carlsberg Research Communications, ISSN: 0105-1938, Vol: 53, Issue: 5, Page: 287-296
1988
  • 47
    Citations
  • 0
    Usage
  • 30
    Captures
  • 1
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    47
    • Citation Indexes
      47
  • Captures
    30
  • Mentions
    1
    • News Mentions
      1
      • 1

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Article Description

Complete solubilisation of barley or malt β-glucan was obtained by extraction with dilute acid. Investigation of the extraction procedure by high performance size exclusion chromatography indicates that originally water insoluble high molecular weight β-glucan is solubilized by degradation to smaller fragments. The fluorescence emitted by the specific complex formed between Calcofluor and β-glucan in solution was used to measure the total β-glucan content of barley and malt samples. Examination of the residues after the extraction with dilute acid by degradation with (1→3)(1→4)-β-D-glucanase and by fluorescence microscopy of Calcofluor stained residues confirmed the complete extraction of the β-glucan. Comparison between the Calcofluor-FIA method and the enzymatic method of McCleary and Glennie-Holmes (20) revealed a high correlation between the values obtained by the two methods. 39 barleys and 57 malts were analysed for β-glucan content with the two methods and linear correlation coefficients of r = 0.977 and r = 0.986, respectively, were found. The system enables a large number of barley or malt β-glucan determinations to be performed within a short period of time with a minimum of manpower. The method is suitable for standard routine analysis in quality control laboratories. © 1988 Carlsberg Laboratory.

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