An improved method for determining β-glucan in wort and beer by use of Calcofluor
Carlsberg Research Communications, ISSN: 0105-1938, Vol: 48, Issue: 5, Page: 505-516
1983
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Article Description
An improved method for the determination of β-glucan in beer and wort employing the fluorochrome Calcofluor is described. Dimethylsulfoxid (20% (v/v)) was used to obtain a reproducible binding ratio of Calcofluor to β-glucan. The formed complex was precipitated by 77% ethanol and separated by centrifugation. The precipitate was dissolved in a glycine/NaOH buffer at pH=10.0 and fluorescence was measured in a spectrofluorometer. The method correlates well with the established ammoniumsulphate precipitation method for β-glucan for a wide range of beer and wort samples. Treatment of beers and worts with pure β-glucanase gave a low background fluorescence, thus indicating a high specificity of the method. From dialysis experiments it was found that low molecular β-glucans<10,000 daltons only gave a slight binding to Calcofluor. The analysis is suitable for routine control in a brewery, allowing an output of at least 50 samples per day per person. © 1983 Carlsberg Laboratory.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0010782964&origin=inward; http://dx.doi.org/10.1007/bf02908693; http://link.springer.com/10.1007/BF02908693; http://www.springerlink.com/index/pdf/10.1007/BF02908693; http://link.springer.com/content/pdf/10.1007/BF02908693; http://link.springer.com/content/pdf/10.1007/BF02908693.pdf; http://link.springer.com/article/10.1007/BF02908693/fulltext.html; https://dx.doi.org/10.1007/bf02908693; https://link.springer.com/article/10.1007/BF02908693; http://www.springerlink.com/index/10.1007/BF02908693
Springer Nature
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