Filamentous bacteriophage display of a bifunctional protein A::scFv fusion
Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology, ISSN: 1073-6085, Vol: 9, Issue: 3, Page: 187-193
1998
- 3Citations
- 2Captures
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Metrics Details
- Citations3
- Citation Indexes3
- CrossRef2
- Captures2
- Readers2
Article Description
Filamentous bacteriophage display is a powerful and widely used technology for the selection of affinity ligands. However, the commonly used phagemid systems result in the production of a population of phage of which those displaying the ligand of interest represent only a small proportion. Through simple dilution and nonspecific binding effects, the presence of large numbers of ligand-free phage reduces the likelihood that weak binders will be successfully selected from a ligand library. To provide a means of avoiding such problems, we have introduced an affinity handle into the phage that permits the purification of ligand displaying phage. The IgG binding domains of Staphylococcus aureus protein A (SpA) were fused to a ligand (single chain Fv[scFv]) which is displayed as a fusion with the phage surface protein ΔpIII. Phage-displaying SpA were separated by affinity chromatography using immobilized human IgG from non-displaying phage and the purified phage were shown to possess functional scFv. Comparisons of fusion proteins in which either the scFv or the affinity handle occupied the amino terminus of the fusion protein showed that, whereas SpA function was unaffected by position, scFv function was compromised when the scFv did not occupy the amino terminus.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0031654508&origin=inward; http://dx.doi.org/10.1007/bf02915792; http://www.ncbi.nlm.nih.gov/pubmed/9718579; http://link.springer.com/10.1007/BF02915792; https://dx.doi.org/10.1007/bf02915792; https://link.springer.com/article/10.1007/BF02915792; http://www.springerlink.com/index/10.1007/BF02915792; http://www.springerlink.com/index/pdf/10.1007/BF02915792
Springer Science and Business Media LLC
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