Molecular profiling using RAPD technique of salt and drought tolerant regenerants of sugarcane
Sugar Tech, ISSN: 0972-1525, Vol: 8, Issue: 1, Page: 63-68
2006
- 23Citations
- 11Captures
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Article Description
Radiation induced and somaclonal variants of sugarcane cv. CoC671 were selected in vitro on different sub-lethal doses of salt (NaCl) and polyethylene glycol 8000 (PEG). The genetic variability among the variants was analyzed using Random Amplified Polymorphic DNA (RAPD) technique with decamer oligonucleotide primers. RAPD analysis resolved 72 scorable bands out of nine primers screened. Primers produced between 6 and 10 amplification products, which ranged 0.1 to 2.0 Kb. The RAPD profile revealed genetic polymorphism between the selected salt and drought tolerant lines from the control plant. Interestingly, the dendrogram also separated SLT (regenerates on salt selection), DRT (regenerates on PEG selection medium) and irradiated regenerants horizontally into two groups suggesting that SLT regenerants completely delineated from others. SLT 171.1 (10 Gy) and SLT 171.1 (20 Gy) were placed together depicting the high similarity between them. The SLT regenerants were placed away from the control supporting the polymorphism revealed by RAPD profiles. This suggests that the irradiated and salt stressed regenerants are better for the selection of mutants. The DRT regenerants were placed close to the control depicting that these did not accumulate much variation as compared SLT and SLT Gy.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33745804287&origin=inward; http://dx.doi.org/10.1007/bf02943744; http://link.springer.com/10.1007/BF02943744; http://www.springerlink.com/index/pdf/10.1007/BF02943744; http://link.springer.com/content/pdf/10.1007/BF02943744; http://link.springer.com/content/pdf/10.1007/BF02943744.pdf; http://link.springer.com/article/10.1007/BF02943744/fulltext.html; https://dx.doi.org/10.1007/bf02943744; https://link.springer.com/article/10.1007/BF02943744; http://www.springerlink.com/index/10.1007/BF02943744
Springer Nature
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