Inhibition of osteoclast differentiation and matrix metalloproteinase production by CD4+CD25+ T cells in mice
Osteoporosis International, ISSN: 0937-941X, Vol: 24, Issue: 3, Page: 1113-1114
2013
- 7Citations
- 4Captures
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Metrics Details
- Citations7
- Citation Indexes7
- CrossRef3
- Captures4
- Readers4
Article Description
To reveal the mechanism of inflammation-accompanied osteoporosis, mouse CD11b cells and CD4+CD25+ T cells were cocultured. Our results showed that CD11b+ monocytes cocultured with CD4+CD25+ T cells generated significantly less osteoclasts than those cocultured with CD4+CD25- T cells. The levels of MMP-7 and 9 in coculture supernatant were decreased significantly when CD11b+ monocytes and CD4+CD25+ T cells were cocultured comparing to the CD4+CD25- T cell group. These results highlight the role of CD4+CD25+ T cells on rheumatic osteoporosis. Introduction: The purpose of this study was to reveal the mechanism of inflammation-accompanied osteoporosis by examining the effect of CD4+CD25+ T cells on osteoclast formation, as well as the production of matrix metalloproteinases (MMP)-2, 3, 7, and 9 from osteoclasts. Methods: Mouse CD11b cells and CD4+CD25+ T cells were isolated using the microbead-based kits. Activated CD11b cells and CD4+CD25+ T were cocultured. CD4+CD25- T cells were used as controls. Osteoclasts were evaluated by counting the average of tartrate-resistant acid phosphatase-positive multinucleated large cells among five high-power fields (×200). After 2 days of coculture, supernatants were harvested for MMP measurement (by ELISA). Results: At day 7, the CD11b+ monocytes cocultured with CD4+CD25+ T cells generated significantly (both p < 0.01) less osteoclasts (3.4 ± 0.8) than those cocultured with CD4+CD25- T cells (16.2 ± 1.3) and those cultured alone (16.2 ± 2.7). The supernatant levels of MMP-7 and 9 were decreased significantly (p < 0.01) when CD11b+ monocytes and CD4+CD25+ T cells were cocultured compared to the other two conditions. Conclusions: The decreased CD4+CD25+ T cells may cause an overproduction of MMP-7 and 9 from osteoclasts, which highlight the role of CD4+CD25+ T cells on rheumatic osteoporosis. © 2012 International Osteoporosis Foundation and National Osteoporosis Foundation.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84878842888&origin=inward; http://dx.doi.org/10.1007/s00198-012-2014-x; http://www.ncbi.nlm.nih.gov/pubmed/22618267; http://link.springer.com/10.1007/s00198-012-2014-x; https://dx.doi.org/10.1007/s00198-012-2014-x; https://link.springer.com/article/10.1007/s00198-012-2014-x
Springer Science and Business Media LLC
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