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Bacteriocinogenic properties of Escherichia coli P2C isolated from pig gastrointestinal tract: purification and characterization of microcin V

Archives of Microbiology, ISSN: 1432-072X, Vol: 200, Issue: 5, Page: 771-782
2018
  • 18
    Citations
  • 0
    Usage
  • 52
    Captures
  • 0
    Mentions
  • 43
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    18
  • Captures
    52
  • Social Media
    43
    • Shares, Likes & Comments
      43
      • Facebook
        43

Article Description

The aim of this study was to isolate and investigate the bacteriocinogenic and probiotic potential of new Gram-negative isolates. Of 22 bacterial isolates from pig intestine and chicken crops, ten isolates had demonstrated a good activity, and the most potent five strains were identified as four E. coli and one as Proteus sp. No virulence factors were detected for E. coli strains isolated from pig intestine. The semi-purified microcins proved to be resistant to temperature and pH variation, but sensitive to proteolytic enzymes. Of particular interest, strain E. coli P2C was the most potent, free of virulence genes and sensitive to tested antibiotics. Purification procedure revealed the presence of a single pure peak having a molecular mass of 8733.94 Da and matching microcin V (MccV). The sequence obtained by LC–MS/MS confirmed the presence of MccV. Purified MccV showed a good activity against pathogenic coliforms, especially E. coli OKH involved in avian colibacillosis. The present study provides evidence that E. coli strains isolated from pig intestine produce microcin-like substances. E. coli P2C is a safe MccV producer that could be a good candidate for its application as novel probiotic strain to protect livestock and enhance growth performance.

Bibliographic Details

Boubezari, Mohammed Tahar; Idoui, Tayeb; Hammami, Riadh; Fernandez, Benoît; Gomaa, Ahmed; Fliss, Ismail

Springer Science and Business Media LLC

Immunology and Microbiology; Biochemistry, Genetics and Molecular Biology

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