Novel cyanobacterial bioreporters of phosphorus bioavailability based on alkaline phosphatase and phosphate transporter genes of Anabaena sp. PCC 7120
Analytical and Bioanalytical Chemistry, ISSN: 1618-2642, Vol: 400, Issue: 10, Page: 3573-3584
2011
- 28Citations
- 34Captures
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Metrics Details
- Citations28
- Citation Indexes28
- 28
- CrossRef23
- Captures34
- Readers34
- 34
Article Description
There is heterogeneity in the way cyanobacteria respond to P starvation and subsequently how they adapt to environments with low or fluctuating P concentrations. In this study, we have fused the promoterless lux operon luxCDABE to the promoter regions of Anabaena sp. PCC 7120 phoA genes putatively encoding alkaline phosphatases, phoA (all2843) and phoA-like (alr5291) and to the promoter region of one operon putatively encoding a high affinity phosphate transporter pst1 (all4575-4572). The self-bioluminescent strains constructed in this way, Anabaena AP (phoA promoter), Anabaena AP-L (phoA-like promoter), and Anabaena PST (pst1 promoter) have been used to study the expression of these genes in response to P starvation and P re-feeding with inorganic and organic phosphate sources. Our data showed that the pst1 promoter was activated at much higher level than the phoA-like promoter following P starvation; however, we did not observe activation of the phoA promoter. The P re-feeding experiments revealed that both strains, Anabaena (A.) PST and A. AP-L could be used as novel bioreporters of P availability in environmental samples. Both strains were used to estimate bioavailable P in environmental samples (fresh- and wastewaters) with a wide range of soluble P concentrations. The results indicated that most of the P in the water samples was in chemical forms available to the cyanobacterium; however there were some differences in the estimates given by both strains as A. PST appeared to be more adequate for the samples with the lowest P load while A. AP-L gave similar or even higher values of P concentrations than those chemically measured in samples with higher P load. [Figure not available: see fulltext.] © 2011 Springer-Verlag.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79960620018&origin=inward; http://dx.doi.org/10.1007/s00216-011-5017-0; http://www.ncbi.nlm.nih.gov/pubmed/21533636; http://link.springer.com/10.1007/s00216-011-5017-0; http://www.springerlink.com/index/10.1007/s00216-011-5017-0; http://www.springerlink.com/index/pdf/10.1007/s00216-011-5017-0
Springer Science and Business Media LLC
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