Preparation, characterization, and application of a new stationary phase containing different kinds of amine groups
Analytical and Bioanalytical Chemistry, ISSN: 1618-2642, Vol: 405, Issue: 25, Page: 8311-8318
2013
- 14Citations
- 5Captures
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Article Description
A new stationary phase containing primary amine and tertiary amine groups was synthesized and its structure was confirmed by infrared spectra, elemental analysis, and zeta potential. This new material was packed into a stainless steel column and the effect of acetonitrile (ACN) content in mobile phase on retention was evaluated using probes of nucleosides, sulfanilamides, and quinolone compounds. This showed hydrophilic interaction, ion interaction, or a mixed-mode retention mechanism for different kinds of solutes, respectively. The effects of the pH and ion strength of the mobile phase were investigated to further understand the retention mechanism of the new stationary phase. The interaction forces caused by the new phase included adsorption, electrostatic function, and ion exchange. The new material could be used as a stationary phase in different high-performance liquid chromatography modes. © 2013 Springer-Verlag Berlin Heidelberg.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84885323531&origin=inward; http://dx.doi.org/10.1007/s00216-013-7243-0; http://www.ncbi.nlm.nih.gov/pubmed/23907685; http://link.springer.com/10.1007/s00216-013-7243-0; https://dx.doi.org/10.1007/s00216-013-7243-0; https://link.springer.com/article/10.1007/s00216-013-7243-0
Springer Science and Business Media LLC
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