Amperometric bienzyme screen-printed biosensor for the determination of leucine
Analytical and Bioanalytical Chemistry, ISSN: 1618-2650, Vol: 406, Issue: 1, Page: 367-372
2014
- 11Citations
- 21Captures
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Article Description
Leucine plays an important role in protein synthesis, brain functions, building muscle mass, and helping the body when it undergoes stress. Here, we report a new amperometric bienzyme screen-printed biosensor for the determination of leucine, by coimmobilizing p -hydroxybenzoate hydroxylase (HBH) and leucine dehydrogenase (LDH) on a screen-printed electrode with NADP and p -hydroxybenzoate as the cofactors. The detection principle of the sensor is that LDH catalyzes the specific dehydrogenation of leucine by using NADP as a cofactor. The product, NADPH, triggers the hydroxylation of p-hydroxybenzoate by HBH in the presence of oxygen to produce 3,4-dihydroxybenzoate, which results in a change in electron concentration at the working carbon electrode, which is detected by the potentiostat. The sensor shows a linear detection range between 10 and 600 μMwith a detection limit of 2 μM. The response is reproducible and has a fast measuring time of 5-10 s after the addition of a given concentration of leucine. © Springer-Verlag Berlin Heidelberg 2013.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84892995193&origin=inward; http://dx.doi.org/10.1007/s00216-013-7443-7; http://www.ncbi.nlm.nih.gov/pubmed/24220759; http://link.springer.com/10.1007/s00216-013-7443-7; https://dx.doi.org/10.1007/s00216-013-7443-7; https://link.springer.com/article/10.1007/s00216-013-7443-7
Springer Science and Business Media LLC
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