Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection: The potential is still growing
Fresenius' Journal of Analytical Chemistry, ISSN: 0937-0633, Vol: 368, Issue: 8, Page: 739-758
2000
- 231Citations
- 246Captures
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Metrics Details
- Citations231
- Citation Indexes231
- 231
- CrossRef150
- Captures246
- Readers246
- 246
Article Description
This article reviews recent advances of carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection. Starting from the paper of Dennis C. Johnson [1] in which the great analytical promise of such a technique was anticipated, a multitude of exciting new research possibilities have recently emerged. The great attractiveness of high-performance anion-exchange chromatography is largely due to its compatibility with such a sensitive, selective and reliable detection method as pulsed amperometry. This very good match between liquid chromatography and electrochemical detection has allowed the determination of carbohydrates in a variety of complex matrices, for instance, foods, beverages, diary and biotechnological products, vegetal tissues, and also in the area of clinical diagnostics. For this reason, the introduction of HPAEC-PAD into regulated methods is becoming increasingly accepted. A comprehensive collection of applications to carbohydrates and samples of interest is given, with special focus on the separation of closely related sugar compounds using dilute alkaline eluents. Advances in pulsed potential waveforms are also discussed, and a comparison with other liquid chromatographic methods addressed. © Springer-Verlag 2000.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034568463&origin=inward; http://dx.doi.org/10.1007/s002160000588; http://www.ncbi.nlm.nih.gov/pubmed/11227559; http://link.springer.com/10.1007/s002160000588; http://www.springerlink.com/index/10.1007/s002160000588; http://www.springerlink.com/index/pdf/10.1007/s002160000588; https://dx.doi.org/10.1007/s002160000588; https://link.springer.com/article/10.1007/s002160000588
Springer Science and Business Media LLC
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