Comparative evaluation of three supplements for helicobacter pylori growth in liquid culture
Current Microbiology, ISSN: 0343-8651, Vol: 60, Issue: 4, Page: 254-262
2010
- 16Citations
- 22Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations16
- Citation Indexes16
- 16
- CrossRef12
- Captures22
- Readers22
- 22
Article Description
Helicobacter pylori, a microaerophilic fastidious bacterium, has been cultured on various plating and broth media since its discovery. Although the agar media can be sufficient for the identification, typing, and antibiotic resistance studies, no secretory antigen of H. pylori can be evaluated in such media. Thus, satisfactory growth of H. pylori in liquid culture which is needed for analysis of secretory proteins without the presence of interfering agents is in demand. We assessed the impact of β-cyclodextrin, Fetal Bovine Serum (FBS), and charcoal as supplements for H. pylori growth. Furthermore, we aimed to identify the most favorable supplement that supports the secretion of the dominant secretory protein, vacuolating cytotoxin (VacA). Five clinical strains were cultured on broth media and the growth, viability, morphology, and protein content of each strain were determined. Our results revealed that β-cyclodextrin supports the growth rate, viability, and cell lysate protein content to the extent similar to FBS. Application of β-cyclodextrin is found to postpone spiral to coccoid conversion up to 72 h of incubation. Although FBS supports a higher VacA protein content, presence of interfering macromolecules in FBS questions its utility particularly for purposes of studying extra cellular proteins such as VacA. This study recommends further application of β-cyclodextrin as a culture supplement with the potential capacity in neutralizing toxic compounds and flourishing the secretion of H. pylori proteins without addition of interfering proteins. © Springer Science+Business Media, LLC 2009.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77952993574&origin=inward; http://dx.doi.org/10.1007/s00284-009-9534-4; http://www.ncbi.nlm.nih.gov/pubmed/19949795; http://link.springer.com/10.1007/s00284-009-9534-4; http://www.springerlink.com/index/10.1007/s00284-009-9534-4; http://www.springerlink.com/index/pdf/10.1007/s00284-009-9534-4; https://dx.doi.org/10.1007/s00284-009-9534-4; https://link.springer.com/article/10.1007/s00284-009-9534-4
Springer Science and Business Media LLC
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