Sciscionella sediminilitoris sp. nov., a Marine Actinomycete Isolated from Cape Rochado, Malaysia, and the Emendations to the Description of the Genus Sciscionella

In this study, we employed a polyphasic approach to determine the taxonomic position of a newly isolated actinomycete, designated SE31, obtained from a sediment sample collected at Cape Rochado, Malaysia. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain SE31 belonged to the family Pseudonocardiaceae and exhibited the highest sequence similarity (98.9%) to Sciscionella marina. Further genomic analysis demonstrated a 93.4% average nucleotide identity and 54.4% digital DNA–DNA hybridization relatedness between strain SE31 and S. marina. The chemotaxonomic characteristics of strain SE31 were typical of the genus Sciscionella, including cell-wall chemotype IV (with meso-diaminopimelic acid as the diagnostic diamino acid, and arabinose and galactose as whole-cell sugars). The identified polar lipids of strain SE31 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, and hydroxyphosphatidymethylethanolamine. The primary menaquinone observed was MK-9(H), and the major cellular fatty acid was iso-C. The genomic DNA size of strain SE31 was determined to be 7.4 Mbp with a G+C content of 68.7%. Based on these comprehensive findings, strain SE31 represents a novel species within the genus Sciscionella, in which the name Sciscionella sediminilitoris sp. nov. is proposed. The type strain of Sciscionella sediminilitoris is SE31 (= DSM 46824 = TBRC 5134).