Variation of particulate carbohydrate pools over time and depth in a diatom-dominated plankton community at the Antarctic Polar Front

Carbohydrate dynamics were studied in an algal community dominated by the diatom Fragilariopsis kerguelensis at the Antarctic Polar Front during austral autumn 1999. Water-extractable mono- and polysaccharide concentrations from the particulate fraction were measured at six depths in the upper 100 m at seven stations along a N-S transect (20°E, 48-50°S) during 2 consecutive days. In addition, field populations were incubated on deck for 18 h at four different light intensities. Polysaccharide concentrations varied between 2.7 and 13.6 μg/l, monosaccharide concentrations between 2.5 and 8.9 μg/l. Near the surface (0-60 m), a diel pattern was observed in the polysaccharide concentration when normalised to the chlorophyll a or the monosaccharide concentration. The deck incubations supported the hypothesis that this pattern resulted from the diurnal accumulation and nocturnal consumption of reserve glucan. More polysaccharides accumulated at high light intensities than at low light intensities, in accordance with the observed decrease in normalised polysaccharide concentration with depth. In addition, the lower concentrations at depth might be explained by consumption: polysaccharides that were accumulated during time spent near the surface were subsequently respired when cells were transported to deeper and dimmer water layers. The variation in carbohydrate pools over time and depth described here must be considered of ecological relevance to phytoplankton in the Southern Ocean subjected to extended periods of darkness (hours to days) due to vertical mixing and advection.