Myelination in coculture of established neuronal and Schwann cell lines
Histochemistry and Cell Biology, ISSN: 0948-6143, Vol: 137, Issue: 6, Page: 829-839
2012
- 29Citations
- 69Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations29
- Citation Indexes28
- 28
- CrossRef24
- Patent Family Citations1
- Patent Families1
- Captures69
- Readers69
- 69
Article Description
Establishing stable coculture systems with neuronal and Schwann cell lines has been considered difficult, presumably because of their high proliferative activity and phenotypic differences from primary cultured cells. The present study is aimed at developing methods for myelin formation under coculture of the neural crest-derived pheochromocytoma cell line PC12 and the immortalized adult rat Schwann cell line IFRS1. Prior to coculture, PC12 cells were seeded at low density (3 × 10 /cm ) and maintained in serum-free medium with N2 supplement, ascorbic acid (50 μg/ml), and nerve growth factor (NGF) (50 ng/ml) for a week. Exposure to such a NGF-rich environment with minimum nutrients accelerated differentiation and neurite extension, but not proliferation, of PC12 cells. When IFRS1 cells were added to NGF-primed PC12 cells, the cell density ratio of PC12 cells to IFRS1 cells was adjusted from 1:50 to 1:100. The cocultured cells were then maintained in serum-free medium with B27 supplement, ascorbic acid (50 μg/ml), NGF (10 ng/ml), and recombinant soluble neuregulin-1 type III (25 ng/ml). Myelin formation was illustrated by light and electron microscopy performed at day 28 of coculture. The stable PC12-IFRS1 coculture system is free of technical and ethical problems arising from the primary culture and can be a valuable tool to study peripheral nerve degeneration and regeneration. © Springer-Verlag 2012.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84865133559&origin=inward; http://dx.doi.org/10.1007/s00418-012-0934-3; http://www.ncbi.nlm.nih.gov/pubmed/22366958; http://link.springer.com/10.1007/s00418-012-0934-3; https://dx.doi.org/10.1007/s00418-012-0934-3; https://link.springer.com/article/10.1007/s00418-012-0934-3; http://www.springerlink.com/index/10.1007/s00418-012-0934-3; http://www.springerlink.com/index/pdf/10.1007/s00418-012-0934-3
Springer Science and Business Media LLC
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